Fig. 7: Quantitative assessment of γ-secretase modulator action on APP in early endosomes and lysosomes. | Nature Communications

Fig. 7: Quantitative assessment of γ-secretase modulator action on APP in early endosomes and lysosomes.

From: Spatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics

Fig. 7

a Overview of experimental design. The indicated cells (n = 3) were left untreated or treated with GSM (BPN-15606) and GSI for 15 h followed by isolation of endosomes and lysosomes together with LMW filtration. Samples were trypsinized and subjected to two sets of 11-plex TOMAHAQ proteomics. b The indicated biological triplicate samples from panel A were subjected to immunoblotting with the indicated antibodies. Quantitative analysis of Aβ peptides in biological triplicate Endo-IP (c) or Lyso-IP (d) samples after LMW filtration (n = 3). Signal-to-noise for MS3 intensities (relative to APP−/− cells, n = 2) is shown. Asterisks refer to two-sided Student’s t-test of DMSO treated samples versus compound treatment: n.s. not significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Exact p values are indicated in the parenthesis. Absolute abundance of individual peptides determined by SIM scans (see “Methods”) is provided below each condition. Absolute abundance of individual peptides determined by SIM scans (see “Methods”) is provided below each condition.

Back to article page