Table 2 Equilibrium binding affinity and kinetic parameters of the interaction between CDTa and CDTb

From: Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile

Buffer

Ligand

ka (×105 M−1s−1)

kd (×10−4 s−1)

KD (nM)

 

WT CDTb

1.44

41.3

28.6

pH 7.4

SPS

1.16

30.4

26.2

 

PSS

1.03

56.7

55.1

 

WT CDTb

1.89

6.19

3.27

pH 7.4, Ca2+

SPS

2.76

5.21

1.89

 

PSS

2.2

10.8

4.93

 

WT CDTb

4.98

7.63

1.53

pH 5.5

SPS

4.41

7.09

1.61

 

PSS

5.84

13.2

2.27

 

WT CDTb

1.52

4.36

2.86

pH 5.5, Ca2+

SPS

1.21

3.26

2.69

 

PSS

1.64

3.76

2.29

  1. All parameters were obtained in the analysis, which were shown in SI Fig. 4.
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