Fig. 5: MALDI-TOF MS data of representative products from refactored RiPP BGCs. | Nature Communications

Fig. 5: MALDI-TOF MS data of representative products from refactored RiPP BGCs.

From: A scalable platform to discover antimicrobials of ribosomal origin

Fig. 5: MALDI-TOF MS data of representative products from refactored RiPP BGCs.The alternative text for this image may have been generated using AI.

Products of the refactored BGCs of a LanII, b LanII-2A, c Las-2, and d Lap-1. Shown are the producing organism, gene diagram for the BGC, sequence of the precursor peptide with the predicted core peptide bolded and the location of PTMs in purple, and MALDI-TOF mass spectrum of the modified peptide. Peptides were analyzed after protease digestion with endoproteinase AspN (a, d) LahT150 (b) or after in vivo production and extraction (c). RRE, RiPP recognition element.

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