Fig. 6: Blocking P300 HAT activity inhibits radiation-induced epigenetic changes and vascular-like conversion in glioma cells.

a Heatmap of logFc expression of HATs in cultured control and radiated gliomaspheres. b Immunoblots of Total and AcH3 in DMSO and C646 treated gliomaspheres. Quantitation of protein level is shown in the graph. N = 3 biological replicates, and * indicates p < 0.05, unpaired two-tailed t-test. c, d Relative expression of endothelial, pericyte and GSC markers in control and radiated gliomaspheres and sorted CD133 + GSC fractions treated with C646. Error bars represent mean ± SD, N = 3 biological replicates. * and ** indicates p < 0.05 and p < 0.005, one-way ANOVA and post hoc t-test. e, f mCherry expression in control and radiated cells treated with C646. Flow-cytometric quantitation of mCherry+ cells. N = 3 biological replicates, * indicates p < 0.05, one-way ANOVA. g Differentially open peak regions (highlighted in black boxes) in vascular genes CDH5, ANGPT1 in control and radiated cells alone or treated with C646. h Fold enrichment of CDH5 and ANGPT1 genomic regions immunoprecipitated with anti-H3K27Ac and control anti-IgG antibodies, normalized to input control N = 3 biological replicates, ** indicates p < 0.005, one-way ANOVA. i LogFC expression (p < 0.05) of endothelial, pericyte and stemness genes in control and radiated glioma cells treated with and without C646. j LogFC expression (p < 0.05) of iGEC and iGPC-enriched growth factors control and radiated glioma cells treated with and without C646. k Heatmap shows significant NES (p < 0.05) of gene sets in control and radiated glioma cells treated with and without C646.