Fig. 4: cFos-tagged dentate granule cells are reactivated during water maze training. | Nature Communications

Fig. 4: cFos-tagged dentate granule cells are reactivated during water maze training.

From: ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning

Fig. 4

a The calcium integrator CaMPARI2 is photoconverted by 405 nm light (violet shading) only in neurons with high intracellular Ca2+. b Experimental timeline: TetTag mice were injected with AAVPHP.eB-TRE-CaMPARI2 and trained in the WM OFF Dox (day 1, cFos tagging, green shading). On day 2, photoconversion light was applied to the DG during WM training or in a novel environment (NE, open field). c Confocal images of DG. Gray is DAPI staining, green is cFos-dependent CaMPARI2 expression, red is immunostaining against photoconverted CaMPARI2 (PC+), cyan is shEGFP immunostaining (cFos+) performed 90 min after the last trial. Note: CaMPARI2 is expressed throughout the cytosol, green nuclear signal is the native shEGFP cFos reporter protein from the TetTag mice. d, e Analysis of CaMPARI2 conversion. Left: Merged images of CaMPARI2 (green) and photoconverted CaMPARI2 (red). Histogram: red bars indicate photoconverted neurons (ratio >0.3). Pie charts indicate % of cFos-tagged (CaMPARI2 expressing) neurons that were photoconverted (red, PC+), cFos+ (cyan), or both (red hatched). d Day 1 and day 2 in water maze (WM-WM, n = 113 cells, 2 mice). e Day 1 WM day 2 novel environment (WM-NE n = 100 cells, 2 mice). Source data are provided as a Source Data file.

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