Fig. 1: Gγ8-GCaMP6s mice enable selective imaging of immature OSN axon odorant responses in glomeruli. | Nature Communications

Fig. 1: Gγ8-GCaMP6s mice enable selective imaging of immature OSN axon odorant responses in glomeruli.

From: Immature olfactory sensory neurons provide behaviourally relevant sensory input to the olfactory bulb

Fig. 1: Gγ8-GCaMP6s mice enable selective imaging of immature OSN axon odorant responses in glomeruli.The alt text for this image may have been generated using AI.

a Schematic of breeding strategy to generate mice expressing GCaMP6s in either immature or mature OSNs under control of the Gγ8 or OMP promoter, respectively, using the tetracycline transactivator system. b MIP of confocal z-stack of coronal OE section from a Gγ8-GCaMP6s mouse showing lack of colocalization with OMP-stained OSNs. c Co-expression of OMP (OMP-ir) in Gγ8-GCaMP6s-expressing OSNs in the septal olfactory epithelium (OE) along the anterior-posterior (A-P) axis. Only 3.0 ± 1.2% of Gγ8-GCaMP6s-expressing neurons co-express OMP (mean ± s.d., n = 4118 Gγ8-GCaMP6s-expressing OSNs from 3 mice). Lines: median, symbols: values for individual mice. Inset shows location of analyzed OE sections. d In vivo 2-photon images of single optical sections showing baseline fluorescence of glomeruli in OMP-tdT-Gγ8-GCaMP6s mice. e Single time point from time series showing peak odorant response for the same optical section as in d. Odorant responses are for stimulation with 2-hexanone, isoamyl acetate, ethyl butyrate and hexanal. d, e Glomeruli from four different mice are shown. Source data are provided as a Source Data file.

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