Fig. 4: Immature OSNs provide information about differences between high odorant concentrations.
a Odorant response amplitudes increase significantly between 0.5–1% concentrations but are similar across higher concentrations for OMP-GCaMP6s mouse-odorant pairs (One-way repeated measures ANOVA. P = 0.001, F1.19,32 = 11.0. Sidak’s multiple comparisons. 0.5% vs. 1%: P < 0.001, t = 5.47. 1% vs. 5%: P = 0.092, t = 2.27. 5% vs. 10%: P = 0.80, t = 0.83. n = 28 mouse-odorant pairs). b Odorant response amplitudes increase significantly between each ascending concentration pair for Gγ8-GCaMP6s mouse-odorant pairs (One-way repeated measures ANOVA. P < 0.001, F1.20,27.7 = 13.2. Sidak’s multiple comparisons. 0.5% vs. 1%: P = 0.002, t = 3.91. 1% vs. 5%: P = 0.030, t = 2.80. 5% vs. 10%: P = 0.023, t = 2.92. n = 28 mouse-odorant pairs). c Response latency is shorter for 1% vs. 0.5 % odorant concentrations but similar between other concentration pairs in OMP-GCaMP6s mice (Bonferroni-corrected one-sample t-tests vs. zero. 0.5–1%: P < 0.001, t = 9.03, n = 10. 1–5%: P = 0.94, t = 0.082, n = 23. 5–10%: P = 0.25, t = 0.60, n = 23). d Response latency decreases as odorant concentration increases in Gγ8-GCaMP6s mice (Bonferroni-corrected one-sample t-tests vs. zero. 0.5–1%: P = 0.016, t = 3.14, n = 9. 1–5%: P = 0.030, t = 2.42, n = 15. 5–10%: P = 0.005, t = 3.24, n = 18). e Similar time to peak across odorant concentrations in OMP-GCaMP6s mice (Bonferroni-corrected one-sample t-tests vs. zero. 0.5–1%: P = 0.88, t = 0.16, n = 10. 1–5%: P = 0.27, t = 1.12, n = 23. 5–10%: P = 0.80, t = 0.26, n = 23). f Time to peak is longer for responses to 10% vs. 5% odorant concentrations but similar across lower concentrations in Gγ8-GCaMP6s mice (Bonferroni-corrected one-sample t-tests vs. zero. 0.5–1%: P = 0.17, t = 1.53, n = 9. 1–5%: P = 0.10, t = 1.75, n = 15. 5–10%: P = 0.017, t = 2.65, n = 18). a–f Lines: mean, symbols: mouse-odorant pairs. All statistical tests were two-tailed. Source data are provided as a Source Data file.
