Fig. 1: The expression of PROM1 in hepatocytes increases after partial hepatectomy.
From: Central role of Prominin-1 in lipid rafts during liver regeneration
A 2/3 partial hepatectomy was performed in 8-week-old male wild-type mice. a The relative mRNA level of PROM1 in sham and PHx livers (n = 3 for sham, n = 5 for 24 h, n = 7 for 48 h, n = 5 for 120 h, p = 0.045). b Immunoblotting for PROM1 in wild-type livers 48 hours after PHx (n = 2 for sham, n = 9 for PHx). c, d Double immunofluorescence for PROM1 and HNF4α (c) or CK19 (d) in sham and PHx livers. e Prom1Cre/ERT2; Rosa26tdTomato mice were generated for lineage tracing of cells expressing PROM1 in the liver. PHx was performed 1 day after tamoxifen injection. The mice were analyzed 7 days after sham (n = 4) or PHx (n = 4). f Representative images of tdTom double immunofluorescence with HNF4α or CK19 in sham and PHx livers. g The percentage of tdTom-expressing cells was statistically determined from total HNF4α- or CK19- expressing cells (p = 6.197×10−6 for HNF4α). Scale bar = 100 µm. Two-sided student t-test; *p < 0.05, ***p < 0.001, n.s, nonsignificant. Data are expressed as the mean ± SEM with individual values. Source data are provided as a Source Data file.
