Fig. 3: Liver-specific deletion of Prom1 in mice impairs liver regeneration after CCl4 injection.
From: Central role of Prominin-1 in lipid rafts during liver regeneration
Eight-week-old male Prom1f/f and Prom1LKO mice were intraperitoneally injected with vehicle (n = 3) or CCl4 (n = 5) for 48 hours. The liver was analyzed by qRT-PCR, immunoblotting and immunofluorescence. a The relative mRNA level for PROM1. The mRNA level of PROM1 was normalized by 18 S rRNA (p = 0.028). b, c Double immunofluorescence for PROM1 and ΗΝF4α (b) or CK19 (c). The experiment was repeated independently three times with similar results. d Immunoblotting for PROM1, Cyclin A and B, and PCNA. e Statistical analysis of the band intensity in d (n = 3, p = 0.033 for Cyclin A, p = 0.029 for Cyclin B, p = 0.005 for PCNA). The band intensity of each protein was normalized to that of β-actin. f Representative H&E staining in the liver. Mitotic cells are indicated by arrows. The experiment was repeated independently three times with similar results. g Double immunofluorescence for Ki-67 and HNF4α. h Statistical analysis of the number of Ki-67-expressing cells (n = 3, p = 4.821 × 10−8). The number of Ki-67-positive cells was normalized to the number of DAPI-stained dots. Scale bar = 100 µm. Two-sided student t-test; *p < 0.05, **p < 0.01, ***p < 0.001. Data are expressed as the mean ± SEM with individual values. Source data are provided as a Source Data file.
