Fig. 6: The first extracellular domain of PROM1 interacts with GP130 and regulates the STAT3 signaling pathway.
From: Central role of Prominin-1 in lipid rafts during liver regeneration
a Structures of PROM1 deletion mutants. EX extracellular domain, TM transmembrane domain, GPI glycosylphosphatidylinositol, GFP green fluorescence protein. b, c Co-immunoprecipitation between each PROM1 mutant and GP130. HEK 293 cells were transfected with various FLAG-tagged PROM1 mutants (1-133, 1-456, 1-812, and PROM1GPI-EX1) or full-length PROM1 (1-856) and His-tagged GP130 for 48 hours. Each experiment was repeated independently three times with similar results. d, e HEK 293 cells were transfected with empty vector (EV) or FLAG-tagged PROM1GPI-EX1 for 48 hours. After serum starvation for 16 hours, HEK 293 cells were treated with 10 ng/ml IL-6 for 0, 15, or 30 minutes. The experiments were independently repeated three times. Immunoblotting for STAT3, P-STAT3 and FLAG (d). Statistical analysis of the band intensity of P-STAT3. The band intensity of P-STAT3 was normalized to that of β-actin. n = 3 independent experiments, p = 3.970 × 10−4 for 15 min, p = 0.002 for 30 min (e). WCL, whole cell lysates; IP, immunoprecipitation; IgG, normal IgG. Two-sided student t-test; **p < 0.01, ***p < 0.001. Data are expressed as the mean ± SEM with individual values. Source data are provided as a Source Data file.
