Fig. 5: Changes in transcriptional behaviour during erythropoiesis.

a Relationship between ON duration and total transcriptional output (area below the curve and above ON threshold for entire imaging period for each cell). LOWESS regression outlines the local relationship between these variables. The inflection point marks a step-change in the gradient of the regression. Cells above the inflection point we call ‘High ON’ cells, while those below are ‘Low ON’. b Example spot intensity traces for individual cells marked by coloured circles in a. Below each panel, thick lines indicate when intensity traces for each cell are above ON threshold (dotted lines). The measured ON duration and output (shaded area) are given for each example cell. (i) ‘basal amplitude’ bursting; (ii) ‘high ON, high amplitude’ bursting; (iii) ‘low ON, high amplitude’ bursting. c Calculated proportions of cells with different bursting behaviours in b at each differentiation stage (defined in Supplementary Fig. 10B, E).