Fig. 1: Cell population landscapes of endometrial pathology.

a Overview of subjects enrolled in this study and representative H&E staining images were shown (n = 5 for each group, scale bar = 500 μm). Normal: normal endometrium; AEH: atypical endometrial hyperplasia; EEC: endometrioid endometrial cancer. b t-SNE plot showed six main cell type clusters of 15 specimens from different stages of endometrial pathology. c Expression patterns of canonical markers and differentially expressed genes (DEGs) of each cell type. Each dot represents a gene, of which the color saturation indicates the average expression level, and the size indicates the percentage of cells expressing the gene. d The percentage of stromal fibroblast and epithelial cells at different stages of endometrial pathology (Source data are provided as a Source Data file). Data are mean ± SEM based on 5 independent biological replicates. Significance was evaluated by comparing it with the normal group (t-test, two-sided; p values are denoted). e Epithelial and stromal cells of different endometrial pathology stages were identified by EPCAM and VIM via immunofluorescence (n = 5 for each group, scale bar = 100 μm). f Spearman correlation test was performed by comparing the expression of marker genes between different endometrial pathology stages (AEH and EEC) and normal sample. g Copy number variation score across different endometrial pathology stages (AEH and EEC) and their cell types.