Fig. 4: Atomic models for the BC domain during catalytic reaction.
From: CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase
a Model of tetrameric LlPC and enlargement of a BC domain. The A sub-domain is colored in cyan, B-subdomain in red, C1-subdomain in green, and C2-subdomain in blue. The B-subdomain interacts mainly with ATP and most of the catalytic residues are located in the A-subdomain. b Overlap of BCopen (blue) and a BCclosed (brown) models. The arrow shows the movement of the B-subdomain (B-lid). c Active site of BCreact. The tethered biotin lays close to the HCO3- molecule which interacts with Glu294, Arg290 and Gln292. ATP is stabilized by two Mg2+ ions. AcCoA stands for acetyl-CoA here and in following figures.
