Fig. 5: Catalytic states at the CT active site.
From: CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase
CryoEM maps together with modeled atomic coordinates are shown focused on the active site of the CT domain. The four states displayed correspond to: a an empty active site (CTempty); b pyruvate bound (CTpyr); c biotin inside the active site and density for oxaloacetate (CTreact); d oxaloacetate at the active site (CToxa). On the right panels the pairs of the corresponding atomic models show the movement of the funnel sub-domain during closing and opening of the active site. Maps are rendered at 2σ density threshold. Abbreviations stand for: Pyr pyruvate; and Oxa oxaloacetate. This notation is used along the figures.
