Fig. 9: Correlation between CTtilt movement and occupancy of the CT site by BCCP.
From: CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase
a Cryo-EM densities of LlPC obtained from 5% of particles at the beginning (left) or the end (right) of vector 4. The volumes have been filtered to improve the density for BCCP domains and they are rendered at 1.1σ of density threshold. Vector 4 corresponds to CTtilt and the movement has been highlighted with red arrows. BCCPs have been marked with asterisks, blue if they interact with BC domain or red if they interact with CT domain. b Overlay of the atomic models for two positions of CTtilt in gray and green. The models were fitted according to the BC of the opposite chain (colored in red/blue). The BCCP was placed in each CT domain according to the CTreact model. The model in gray/red shows an 8 Å gap in the loop connecting BCCP and PT domains.
