Fig. 3: Cryo-EM analysis of nanodisc-embedded gp145 in complex with the Fab of anti-MPER bnAb 4E10.
From: Dynamic HIV-1 spike motion creates vulnerability for its membrane-bound tripod to antibody attack
a Representative negative-stain EM image of nanodisc-embedded gp145 after incubation with 4E10 Fab (left) and 2D-class averages showing gp145 with one to three bound 4E10 Fabs (right). Scale bar: 50 nm; side length of averages: 34 nm. b Cryo-EM maps of gp145 with one, two, and three bound 4E10 Fabs. The maps were automatically segmented with the “color zone” command in Chimera. The ectodomain and nanodisc are colored in light gray and Fabs 1 to 3 are colored in yellow, gold, and brown, respectively. Asterisks indicate density representing the PG9 Fab used for purification.
