Fig. 3: Quantitative analysis using CAPTORs. | Nature Communications

Fig. 3: Quantitative analysis using CAPTORs.

From: Library adaptors with integrated reference controls improve the accuracy and reliability of nanopore sequencing

Fig. 3: Quantitative analysis using CAPTORs.

a Schematic diagram illustrates the preparation of a master mixture that constitutes a staggered dilution of CAPTORs. Use of the staggered CAPTORs master mix generates an internal quantitative reference ladder that can be used to measure technical variation and improve normalisation between samples. b Scatter plot compares the observed CAPTOR count relative to their expected concentrations, forming a staggered reference ladder to evaluate the quantitative performance of an individual library. 95% confidence intervals of line of best fit are indicated. c Scatter plot compares the quantitative reference ladder formed by the CAPTORs relative to the read counts for synthetic microbes within a mock microbial community sample. d Violin plot shows the sequencing error for CAPTORs at different concentrations, illustrating the impact of sequencing coverage on accuracy. Source data are provided as a Source Data file.

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