Fig. 3: Cold-responsive RNA G-quadruplex in 3′-UTR represses mRNA decay.
From: RNA G-quadruplex structure contributes to cold adaptation in plants
a Comparison of translation efficiency (TE) at 22 °C and 4 °C, for transcripts without RG4 (nonRG4), with RG4 in 5′-UTR, with RG4 in CDS, and with RG4 in 3′-UTR. The number of transcripts, n = 465, 6, 531, 36, for nonRG4, 5′-UTR, CDS and 3′-UTR respectively. TE was calculated based on three independent biological replicates. b Comparison of folding score differences (4–22 °C) between RG4 located in different genic regions. The number of RG4 sites, n = 9, 1421, 57 for 5′-UTR, CDS, and 3′-UTR, respectively; P values by two-sided Student’s t test. c Schematic of determining RNA stability in Arabidopsis at 22 °C and 4 °C, by transcriptional arrest analysis with cordycepin incubation. d RNA abundance curves at 22 °C and 4 °C for transcripts with cold-responsive RG4s, otherwise in a. Data are presented as mean values ±SE of different transcripts. RNA abundance was calculated based on three independent biological replicates. e Comparison of the decay rate of transcripts with cold-responsive RG4s at 22 °C and 4 °C, otherwise in a. f Comparison of decay rate differences (4–22 °C) on transcripts with cold-responsive RG4s. P value by two-sided Student’s t test, otherwise in a. g Line plot showing relative mRNA abundance at 4 °C versus 22 °C for transcripts with cold-responsive RG4s. P values by Student’s t test, otherwise in a.
