Fig. 5: Cell surface protein expression and intracellular localization of NA Mos99 mutants.
From: Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidase
a Cell surface expression of HA-tagged Mos99 mutants was analyzed by flow cytometry. b Confocal microscopy result of HA-tagged Mos99 mutants. Blue (DAPI), Green (Calnexin, ER), Magenta (NA). The orange box highlights the zoomed in region. Scale bar for the large image is 5 µm and for zoomed in image is 2 µm. Below each micrograph is a cytofluorogram, in which each datapoint represents a pixel. The Pearson correlation coefficient (r) between the green intensity (Calenxin, ER) and magenta intensity (NA) across all pixels in each image is indicated. The result of a representative experiment out of two independent replicates is shown.
