Fig. 1: Additional quantifications of changes in BiP levels from MERS-CoV or SARS-CoV-2-infected cells in the presence or absence of thapsigargin (Tg) reported by Shaban et al.¹.

a Graphs with split Y-axes showing the quantification of BiP and β-actin protein bands in HuH7 cells infected with MERS-CoV (MOI of 0.5) or Vero E6 cells infected with SARS-CoV-2 (MOI of 0.5) for 12 h or 24 h as previously presented in Supplementary Fig. 3 of Shaban et al.,¹. Data points represent three biologically independent experiments. Representative immunoblots are provided in Fig. 4 g (MERS-CoV) and Fig. 4 h (SARS-CoV-2) of Shaban et al.,¹. BiP levels were quantified relative to the untreated control. Bar graphs show means ± s.d.; asterisks indicate significant changes (*p ≤ 0.05) obtained by two-tailed ratio-paired t-tests; ns indicates non-significant changes. b Normalized protein intensity values of BiP expression were determined by label-free LC-MS/MS-based quantification of tryptic peptides derived from total cell extracts of uninfected HuH7 cells (−), cells infected with MERS-CoV (MOI of 3) for 12 h or 24 h (left graphs), or Vero E6 cells infected with SARS-CoV-2 (MOI of 3) for 12 h or 24 h (right graphs), in the presence or absence of thapsigargin (1 µM). At least 53 (Huh7 cells) or 78 (Vero E6 cells) unique peptides were obtained, representating a sequence coverage of BiP ranging from 60.1 to 69.6%. Raw data can be retrieved using the following link: 10.6019/PXD021222. Floating bars show minimum to maximum values and means. Data points represent two biological and three technical replicates. Asterisks indicate p values (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001) obtained by ordinary one-way ANOVA. c Graphs with split Y-axes showing the quantification of BiP and β-actin protein bands in HuH7 cells infected with HCoV-229E (MOI of 3), MERS-CoV (MOI of 3) or Vero E6 cells infected with SARS-CoV-2 (MOI of 3) for 24 h as previously presented in Fig. 9b of Shaban et al.,¹. Data points represent four biologically independent experiments. Representative immunoblots are shown in Fig. 9a of Shaban et al.,¹. BiP levels were quantified relative to the untreated control. Bar graphs show means ± s.d.; asterisks indicate significant changes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) obtained by two-tailed ratio-paired t-tests; ns indicates non-significant changes.