Fig. 4: Ptp10D and EGFR recycling is facilitated by retromer and retriever complexes, respectively. | Nature Communications

Fig. 4: Ptp10D and EGFR recycling is facilitated by retromer and retriever complexes, respectively.

From: WASH activation controls endosomal recycling and EGFR and Hippo signaling during tumor-suppressive cell competition

Fig. 4: Ptp10D and EGFR recycling is facilitated by retromer and retriever complexes, respectively.The alternative text for this image may have been generated using AI.

a–e Immunochemistry analysis for aPKC and Ptp10D. Top images show xy confocal sections of eye disc bearing GFP-labelled scrib−/− (a), scrib−/− Vps26RNAi (b), scrib−/− SNX27RNAi (c), scrib−/− Vps29RNAi (d) and scrib−/− SNX17RNAi (e) clones immunostained with anti-aPKC (gray), anti-Ptp10D (magenta), anti-GFP (green) and DAPI (blue); bottom images show xz cross sections. Dashed lines in the top right images with all the channels mark the positions of the cross-sections in the bottom images. Note that the interface Ptp10D and aPKC of scrib−/− Vps26RNAi, scrib−/− SNX27RNAi and scrib−/− Vps29RNAi clones were disrupted (yellow arrowheads). f–j Immunochemistry analysis for EGFR. Top images show xy confocal sections of eye disc bearing GFP-labelled scrib−/− (f), scrib−/− Vps26RNAi (g), scrib−/− SNX27RNAi (h), scrib−/− Vps29RNAi (i) and scrib−/−−/− SNX17RNAi (j) clones immunostained with anti-EGFR (magenta), anti-GFP (green) and DAPI (blue); bottom images show xz cross sections. Dashed lines in the top right images with all the channels mark the positions of the cross-sections in the bottom images. Note that EGFR increased inside the clones of scrib−/− Vps29RNAi and scrib−/− SNX17RNAi (yellow stars). k–o Eye-discs bearing GFP-labelled scrib−/− (k), scrib−/− Vps29RNAi (l), scrib−/− Vps26RNAi (m), scrib−/− SNX27RNAi (n) and scrib−/− SNX17RNAi (o) clones immunostained with anti-Cic (gray) and anti-GFP (green). p Quantification of total GFP + area (%) in genotypes shown in scrib−/− (n = 14, number of eye discs), scrib−/− Vps29RNAi (n = 13) and scrib−/− Vps26RNAi (n = 9). q Quantification of Capicua signal in scrib−/− (n = 16, number of clones), scrib−/− Vps29RNAi (n = 15) and scrib−/− Vps26RNAi (n = 23) clones. r Quantification of total GFP+ area (%) in genotypes shown in scrib−/− (n = 22, number of eye discs), scrib−/− SNX17RNAi (n = 24) and scrib−/− SNX27RNAi (n = 23). s Quantification of Capicua signal in scrib−/− (n = 31, number of clones), scrib−/− SNX17RNAi (n = 32) and scrib−/− SNX27RNAi (n = 29) clones. p-s Data are mean ± s.e.m; **P < 0.005, ***P < 0.0005, ****P < 0.0001 by two tailed unpaired Mann-Whitney U-test. All scale bars are 20 μm.

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