Fig. 1: Comparison of PCR and SHARP. | Nature Communications

Fig. 1: Comparison of PCR and SHARP.

From: Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics

Fig. 1: Comparison of PCR and SHARP.The alternative text for this image may have been generated using AI.

a PCR alternates the temperature between high (≈98 °C) to melt the DNA duplex and low (45–65 °C) to replicate the complementary strand by a DNA polymerase. b SHARP uses PcrA M6 helicase and SSB to open the duplex and enable primer binding, followed by Bst-LF polymerase to replicate the strand. SHARP occurs at a constant temperature. Created with BioRender.com.

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