Fig. 3: Other SHARP products. | Nature Communications

Fig. 3: Other SHARP products.

From: Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics

Fig. 3: Other SHARP products.The alternative text for this image may have been generated using AI.

a 3 kbp SHARP amplicon. b 6 kbp SHARP amplicon. c 200 bp SHAR amplicon using λDNA as a template. d SHARP doubling time estimate for 200 bp product. e Detection limit versus the amplicon length. The area above the detection limit is shaded in gray and labeled detectable. f Doubling time shows that short amplicons replicate at the highest rate. The standard deviation of the copy number in panels a and b is 4%, while for panel c, it is approximated as \(3.8\%+1 / \sqrt{{copy}}\), see Methods for details. Source data are provided as a Source Data file.

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