Fig. 5: Applications of SHARP. | Nature Communications

Fig. 5: Applications of SHARP.

From: Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics

Fig. 5: Applications of SHARP.The alternative text for this image may have been generated using AI.

a Transforming SHARP-made plasmid to E. coli. b SHARP amplifies sequence prone to forming secondary structure (CAG repeats) at different SSB concentrations. c SHARP kinetics curves at different temperatures, inset shows detection times. d SHARP product at different temperatures. e SHARP at 37 °C with Bsu and Klenow Exo- DNAPs at variable SSB concentration. The standard deviation of the template copy number and SSB concentration is 4%, see Methods for details. Source data are provided as a Source Data file.

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