Table 1 SHARP components for 40 \(\mu\)L reaction volume
Component 1 (2X): | \(\mu\)L | Component 2 (2X): | \(\mu\)L |
|---|---|---|---|
Nuclease free water | 9 | Nuclease free water | 11.1 |
10X Buffer | 2 | 10X Buffer | 2 |
EvaGreen (20X) | 2 | DTT (100 mM) | 4 |
dNTPs (10 mM each) | 2 | Bst-LF (1.5 mg mL−1) | 0.2 |
ATP (100 mM) | 2 | SSB (9 mg mL−1) | 0.5 |
Forward primer (10 uM) | 1 | PcrA M6 (0.2 mg mL−1) | 2 |
Reverse primer (10 uM) | 1 | Thermostable PPase (100 units mL−1) | 0.2 |
Template (variable conc.) | 1 | ||
Total | 20 | Total | 20 |
