Fig. 4: Mitochondrial transcripts are altered upon ablation of mtRF1 or mtRF1a. | Nature Communications

Fig. 4: Mitochondrial transcripts are altered upon ablation of mtRF1 or mtRF1a.

From: Human mtRF1 terminates COX1 translation and its ablation induces mitochondrial ribosome-associated quality control

Fig. 4: Mitochondrial transcripts are altered upon ablation of mtRF1 or mtRF1a.The alternative text for this image may have been generated using AI.

a, b RNA was isolated from wild-type (WT) cells, mtRF1−/− and mtRF1a−/− and subjected to northern blot analysis. Mitochondrial mRNAs encoding for COX1 (MTCO1), COX2 (MTCO2) and CYTB (MTCYTB) as well as the mt-rRNAs 12 S rRNA (MTRNR1) and 16 S rRNA (MTRNR2) were detected using specific probes. 18 S rRNA was used as loading control. RNA levels are calculated relative to WT (dashed line) and individual data points are indicated as circles. Statistical analysis was performed as two-sample one-tailed Student’s t-test with n = 3 biologically independent samples and shown as mean ± SEM. Significance was defined as *p ≤ 0.05 ; **p ≤ 0.01 ; ***p ≤ 0.001. c, d RNA was isolated from WT, mtRF1−/− and mtRF1a−/− treated with chloramphenicol (CAM, 50 µg/ml) for 24 h as indicated. Northern blot and statistical analysis were performed as in a, b. e Isolated RNA from WT and mtRF1−/− mitochondria was subjected to NanoString analysis. 18 S and 5 S rRNA were used as controls. The relative RNA levels of WT are indicated as dashed line. Statistical analysis was performed as two-sample one-tailed Student’s t-test with n = 3 biologically independent samples and shown as mean ± SEM. Significance was defined as ***p ≤ 0.001. Source data are provided as a Source Data file.

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