Fig. 6: The hRQT complex dissociates collided ribosomes with K63-linked polyubiquitin chains on uS10. | Nature Communications

Fig. 6: The hRQT complex dissociates collided ribosomes with K63-linked polyubiquitin chains on uS10.

From: A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation

Fig. 6

A–D In vitro hRQT complex-mediated subunit disassociation assay of K63-linked polyubiquitinated ribosomes. Isolated RNCs generated by XBP1u staller mRNA were ubiquitinated by ZNF598 with either Ub-K63R (A, B) or Ub-K63only (C, D). The ubiquitinated RNCs were incubated with (B, D) or without (A, C) the hRQT complex. After hRQT-mediated splitting reactions, samples were subjected to ultracentrifugation through sucrose density gradients and gradient fractions were analyzed by western blotting using indicated antibodies. We obtained essentially the same results in at least three independent experiments.

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