Fig. 4: Myeloid Redd1 deficiency prevents HFD-induced meta-inflammation and metabolic dysregulation.
From: REDD1 promotes obesity-induced metabolic dysfunction via atypical NF-κB activation
a Weight gain in Redd1fl/fl (Rfl/fl) and Redd1ΔLysM (RΔLysM) mice fed HFD for 16 weeks (n = 5 per group). b Measurement of fat (eWAT + iWAT) mass in HFD-fed Redd1fl/fl and Redd1ΔLysM mice (n = 5 per group). c Representative images showing perilipin (green) and F4/80 (purple) staining in the eWAT of HFD-fed Redd1fl/fl and Redd1ΔLysM mice (n = 5 per group). Scale bar, 100 μm. d NF-κB activity in the eWAT from HFD-fed Redd1fl/fl and Redd1ΔLysM mice (n = 5 per group). e Plasma levels of inflammatory cytokines in HFD-fed Redd1fl/fl and Redd1ΔLysM mice (n = 5 per group). f Fasting plasma levels of glucose and insulin in HFD-fed Redd1fl/fl and Redd1ΔLysM mice (n = 5 per group). g Calculation of HOMA-IR scores in HFD-fed Redd1fl/fl and Redd1ΔLysM mice (n = 5 per group). h Assessment of GTT and ITT in HFD-fed Redd1fl/fl and Redd1ΔLysM mice fasting for 12 and 6 h, respectively (n = 5 per group). i, j Representative western blots of the insulin-responsive phosphorylation of IRS-1 and Akt in the eWAT and skeletal muscle (i) and phosphorylation of Akt and FOXO1 in the liver (j) of NC- or HFD-fed Redd1fl/fl and Redd1ΔLysM mice (n = 3). k Relative expression levels of G6pc, Pck1, and Fbp1 in the liver of HFD-fed Redd1fl/fl and Redd1ΔLysM mice compared with NC-fed mouse groups (n = 5 per group). Bar graphs represent mean ± s.e.m. Statistical significance was calculated using an unpaired two-tailed t-test. Source data are provided as a Source Data file.
