Table 1 Comparison of canthaxanthin dihedral angles observed in OCP structures in the three states: Dark, I1 and I4. Dihedral angles were measured using atomic PDB coordinates for carbon atoms
From: Light activation of Orange Carotenoid Protein reveals bicycle-pedal single-bond isomerization
Dark (this study) | 1 m (this study) | 10 m (this study) | Syn OCPCAN (4XB5) | Syn OCPECH (5TUX) | Arth OCPhECH (5UI2 -CA) | Arth OCPhECH (5UI2 -CB) | |
|---|---|---|---|---|---|---|---|
C5-C6-C7-C8 (β1 ring) | 153 | 157.8 | −35.6 | 116.3 | 131.8 | 118.5 | 131.9 |
C6-C7-C8-C9 | 160 | 163.1 | 159.9 | 175.4 | 176.7 | 172.8 | 174.7 |
C7-C8-C9-C10 | 167 | 160.4 | 167 | −155.3 | −168.7 | −163.7 | −176.8 |
C8-C9-C10-C11 | 164.9 | 162.4 | 164.9 | 175.8 | 174.9 | 177.9 | 171.1 |
C9-C10-C11-C12 | −176.1 | −166.1 | −176.2 | 178.8 | −179.5 | 165.2 | 173.6 |
C10-C11-C12-C13 | 176.9 | −172.8 | 177.0 | 174.4 | 173.7 | 175.4 | 175.5 |
C11-C12-C13-C14 | 177.6 | 163 | 177.7 | 167.8 | 170 | 177.5 | 175.9 |
C12-C13-C14-C15 | 172.7 | 175.4 | 172.7 | 173 | 172.8 | 171 | 170 |
C13-C14-C15-C15’ | 175 | −176.2 | 175 | −171.4 | 177.8 | −175.7 | 171.5 |
C14-C15-C15’-C14’ | 172.4 | 144.9 | 172.4 | 172.7 | 171.8 | 170.7 | 171.4 |
C15-C15’-C14’-C13’ | −175.2 | 163.8 | −175.2 | 169.8 | −178.7 | 179.6 | −168.2 |
C15’-C14’-C13’-C12’ | 171.5 | −177.5 | 171.5 | 173 | 172.2 | 172.8 | 169.8 |
C14’-C13’-C12’-C11’ | −170.9 | −148.1 | −170.9 | −153.3 | −165.9 | −161.6 | 171.4 |
C13’-C12’-C11’-C10’ | 172 | 171 | 172 | 173.1 | 172.8 | 174.8 | 170.9 |
C12’-C11’-C10’-C9’ | −175.9 | 167.5 | −175.9 | 171.9 | −179.2 | 170.3 | −158.8 |
C11’-C10’-C9’-C8’ | 172.9 | 173.3 | 172.8 | 175.2 | 174.8 | 174 | −179.7 |
C10’-C9’-C8’-C7’ | 178.4 | −23 | 178.3 | −160.3 | −175.1 | −170.2 | 174.9 |
C9’-C8’-C7’-C6’ | 176.3 | −171.6 | 176.3 | 177.6 | 178.5 | 177.3 | 178.6 |
C8’-C7’-C6’-C5’ (β2 ring) | −45.3 | 145.1 | −45.3 | −72.1 | −58.6 | −66 | −51.9 |
RMSD | 0.436 | 0.706 | 0.436 |
