Fig. 5: The GARR-1 N and C terminal RG/RGG domains contribute to sub-nucleolar organization in the germline. | Nature Communications

Fig. 5: The GARR-1 N and C terminal RG/RGG domains contribute to sub-nucleolar organization in the germline.

From: RG/RGG repeats in the C. elegans homologs of Nucleolin and GAR1 contribute to sub-nucleolar phase separation

Fig. 5

a GARR-1 WT localizes to the FC of pachytene germ cell nucleoli. GARR-1ΔNRGG and GARR-1ΔCRGG show a loss of this compartmentalization b and decreased CV compared to WT (n = 152 nucleoli from 8 GARR-1 WT worms, 135 nucleoli from 8 GARR-1ΔNRGG worms, and 84 nucleoli from 6 GARR-1ΔCRGG worms). ****p < 0.0001. c LPD-6ΔRGG protein does not show differences in nucleolar organization compared to WT. Both LPD-6 WT and LPD-6ΔRGG are less homogenously dispersed than free GFP. d There is no significant difference in CV between LPD-6 and LPD-6ΔRGG, but both have a significantly greater CV compared to free GFP (n = 351 nucleoli from 14 LPD-6 WT worms, 335 nucleoli from 14 LPD-6ΔRGG worms, and 159 nucleoli from nine DUP218 (GLH-1::T2A::sGFP1-11) worms). ****p < 0.001. b, d One-way ANOVA with Tukey’s multiple comparisons test. Points represent individual nucleoli and means ± SD are shown.

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