Fig. 7: The effects of RG/RGG domain deletion on fertility and development. | Nature Communications

Fig. 7: The effects of RG/RGG domain deletion on fertility and development.

From: RG/RGG repeats in the C. elegans homologs of Nucleolin and GAR1 contribute to sub-nucleolar phase separation

Fig. 7

a NUCL -1ΔRGG worms show no defect in brood size (Generation 5–10: 20 °C n = 19 WT and 21 ΔRGG worms, 25 °C n = 20 WT and 21 ΔRGG worms. Generation 80–90: 15 °C n = 19 WT, 18 ΔRGG worms, 20 °C n = 23 WT and 22 ΔRGG worms, 25 °C n = 25 WT and 24 ΔRGG worms). b NUCL-1 ΔRGG worms have normal vulval maturity (n = 103 WT and 134 ΔRGG worms). c LPD-6ΔRGG worms show no defect in brood size (15 °C: n = 20 WT and 18 ΔRGG worms, 20 °C n = 22 WT and 22 ΔRGG worms, 25 °C n = 24 WT and 22 ΔRGG worms). d GARR-1ΔNRGG and GARR-1ΔCRGG worms have a smaller brood size at 20 °C than GARR-1 WT and produce no progeny at 15 °C (15 °C n = 24 WT, ΔNRGG and ΔCRGG worms, 20 °C n = 23 WT, 20 ΔNRGG, and 21 ΔCRGG worms, 25 °C n = 23 WT, 24 ΔNRGG, and 23 ΔCRGG worms). ****p = <0.0001. e Percentage of fertile worms is decreased in GARR-1ΔNRGG and GARR-1ΔCRGG, especially at 15 °C (15 °C: n = 6 WT, 7 ΔNRGG, and 6 ΔCRGG; 20 °C: n = 6 WT, 6 ΔNRGG, and 5 ΔCRGG; 25 °C: n = 7 WT, 6 ΔNRGG, and 6 ΔCRGG experimental replicates of 20 worms each). 15 °C ****p < 0.0001; 25 °C GARR-1WTvs GARR-1ΔCRGG p = 0.0016, GARR-1ΔNRGG vs GARR-1ΔCRGG p = 0.0026. f GARR-1ΔNRGG and GARR-1ΔCRGG worms have delayed vulval maturation (n = 46 WT, 107 ΔNRGG, and 83 ΔCRGG worms). ****p = <0.0001. g GARR-1ΔNRGG and GARR-1ΔCRGG embryos show delayed hatching (18 h: n = 12 WT, 12 ΔNRGG, and 10 ΔCRGG; 24 h: n = 12 WT, 12 ΔNRGG, and 7 ΔCRGG experimental replicates of 20 worms each). ****p = <0.0001. h 5 generations after NUCL-1 deletion worms have a subtly decreased brood size at 25 °C (15 °C: n = 22 WT and 21 ΔNUCL-1 worms, 20 °C n = 30 WT and 30 ΔNUCL-1 worms, 25 °C n = 21 WT and 21 ΔNUCL-1 worms). p = 0.0064. i ΔNUCL-1 worms show delayed vulval maturation at 20 °C (n = 64 WT, 55 5th Gen. ΔNUCL-1, and 64 40th Gen. ΔNUCL-1 worms). ****p = <0.0001. j Although ΔNUCL-1 and LPD-6ΔRGG worms have normal brood sizes, double mutant ΔNUCL-1; LPD-6ΔRGG worms produce significantly fewer progeny (n = 11 WT, 11 ΔNUCL-1, 11 LPD-6 ΔRGG, and 12 ΔNUCL-1; LPD-6ΔRGG worms. ****p = <0.0001. a, c–j One-way ANOVA with Tukey’s multiple comparisons test. b Unpaired, two-tailed t-test with Welch’s correction. All data are shown as means ± SD.

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