Fig. 4: X-ray damage in collagen as compared to the lateral beam size.

a Accumulated damage (DV) as a function of radiation dose: with decreasing lateral beam diameter (Pinhole-ID: 100 μm (black rectangles), 20 μm (cyan triangles), 10 μm (magenta diamonds), and 5 μm (blue stars)) the damage extent (V) increases relative to the beam size (=100%) to ~120% (k = 0.33% s−1), ~150% (k = 0.07% s−1), ~240% (k = 0.02% s−1) and ~ 260% (k = 0.02% s−1), respectively, after exposure for 320 s. In all cases, the damaged volume substantially exceeds the beam volume inside the bone (indicated with a line parallel to the x-axis). b Accumulated damage when irradiated for different exposure times (t): 80 s, 160 s, and 320 s with a 20 μm pinhole. Black dots refer to relative damaged volume with respect to the beam size, as determined by SHG. Magenta lines depict the results of the model function Z(w, Δt) for the case of ζV ≈ 1.