Fig. 1: Single-nucleus transcriptional profiling on human ADPKD kidneys. | Nature Communications

Fig. 1: Single-nucleus transcriptional profiling on human ADPKD kidneys.

From: Defining cellular complexity in human autosomal dominant polycystic kidney disease by multimodal single cell analysis

Fig. 1: Single-nucleus transcriptional profiling on human ADPKD kidneys.The alternative text for this image may have been generated using AI.

a Overview of experimental methodology. n = 8 human ADPKD kidneys and n = 5 control kidneys were analyzed with snRNA-seq and snATAC-seq. Batch effect on the integrated datasets was corrected with Harmony. See Method section for detail. b UMAP plot of integrated snRNA-seq dataset with annotation by cell type (left) or disease condition (right). PT proximal tubule, PEC parietal epithelial cells, TAL thick ascending limb of Henle’s loop, DCT distal convoluted tubule, CNT_PC connecting tubule and principal cells, ICA Type A intercalated cells, ICB Type B intercalated cells, PODO podocytes, ENDO endothelial cells, FIB fibroblasts, LEUK leukocytes, URO uroepithelium. c Dot plot of snRNA-seq dataset showing gene expression patterns of cluster-enriched markers for ADPKD or control kidneys. For LEUK and URO1/2 clusters, data from ADPKD kidneys were shown. The diameter of the dot corresponds to the proportion of cells expressing the indicated gene and the intensity of the dot corresponds to average expression relative to all cell types.

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