Fig. 3: Effects of EMC10 ablation and scEMC10 overexpression on obesity and metabolic homeostasis.

A Body weights of male (WT, black circle), and KO (red square) on C57BL/6 background on HFD (n = 6 & 7 per group). B Tissues (iWAT, eWAT, BAT, liver) weight from male WT (open), and KO (red) mice fed with 12-wks of HFD (n = 6 per group). C Body composition of male WT (open) and KO (red) mice fed HFD by DEXA (n = 6 per group). D Glucose tolerance (left) and insulin tolerance (right) in male WT (black diamond), and KO (red square) mice fed with 12-wks of HFD (n = 8 & 7 per group). Plasma glucose and insulin (E); leptin and adiponectin (F); triglyceride (TG), cholesterol (CHO), and non-esterified free fatty acid (NEFA) (G), in male WT (open), and KO (red) mice fed with 12-wks of HFD in the fed or overnight fasted states (n = 6 per group). H Representative images of H&E-stained sections of livers from male WT and KO mice fed with 12-wk of HFD. Scale bar, 100 um. I TG content of liver from male WT (open) and KO (red) mice fed with 12-wks of HFD (n = 6 per group). J Body weights of male C57BL/6 mice expressing LacZ control or hscEMC10 via tail-vein AAV transduction after 20-wks of chow diet (CD) (n = 6 per group). K Tissues (iWAT, eWAT, retroperitoneal (retroWAT), BAT, liver) weight of male C57BL/6 mice expressing LacZ control or hscEMC10 via tail-vein AAV transduction after 20-wks of CD (n = 6 per group). L Glucose tolerance (left) and insulin tolerance (right) of male C57BL/6 mice expressing LacZ control or hscEMC10 via tail-vein AAV transduction after 20-wks of CD (n = 6 per group). M Plasma insulin, leptin, and adiponectin of male C57BL/6 mice in the fed state expressing LacZ control or hscEMC10 via tail-vein AAV transduction after 20-wks of CD (n = 6 per group). All data are presented as mean +/− SEM. Statistical significance was assessed by two-sided Student’s t test and significant differences were indicated with p values. Source data are provided in the Source Data file.