Fig. 4: EMC10 ablation promotes adipose tissue oxygen consumption and whole-body energy expenditure.

A Oxygen consumption (VO2) (left) and carbon dioxide production (VCO2) (right), B Heat production analyzed by indirect calorimetry for 48 h in male WT (black circle) or KO (red square) mice after fed with 12-wks of HFD (n = 8 per group). C Rectal temperature measured for male WT (black) and KO (red) mice fed HFD at room temperature (n = 8 per group). D Energy expenditure analyzed with ANCOVA using lean mass as covariate for WT (black circle) or KO (red square) mice (n = 8 per group). E Respiratory exchange ratio (RER) analyzed by indirect calorimetry in male WT (black circle) and KO (red square) mice fed with HFD (n = 6 per group). F Atgl, Hsl, Adrb3, Glut4, Fasn, Srebp1c, Pgc1a, Ucp1, and Tfam mRNA in BAT from male WT (balck) or KO (red) mice after fed with 12-wks of HFD (n = 6 per group). G Atgl, Hsl, Adrb3, Pgc1a, Ucp1, and Tfam mRNA in iWAT from male WT (balck) or KO (red) mice after fed with 12-wks of HFD (n = 6 per group). H Pgc1a, Ucp1, and Tfam mRNA in BAT and iWAT from male WT (black) or KO (red) mice after fed with 12-wks of LFD (n = 6 per group). I Oxygen consumption in BAT (left) and iWAT (right) from male WT (black) or KO (red) mice fed with 12-wks of LFD. (n = 6 per group). J Body weights of male (WT, black circle), and KO (red square) on HFD at 30 °C (n = 5 & 6 per group). K Oxygen consumption of male WT (black circle) and KO (red square) before and after CL316, 243 (0.1 mg/kg) stimulation (n = 5 & 4 per group). All data are presented as mean +/− SEM. Student’s t test was used for statistical analysis. Statistical significance was assessed by two-way ANOVA followed with Bonferroni’s multiple comparisons test (A, B and E), two-sided Student’s t test (C, F–K) and significant differences were indicated with p values. Source data are provided in the Source Data file.