Fig. 4: Cell and tissue compatibility. | Nature Communications

Fig. 4: Cell and tissue compatibility.

From: Modular stimuli-responsive hydrogel sealants for early gastrointestinal leak detection and containment

Fig. 4: Cell and tissue compatibility.The alternative text for this image may have been generated using AI.

a Lactate dehydrogenase (LDH) release cytotoxicity assay of fibroblasts treated with conditioned cell medium as a function of perfusion iterations of the sealant patches with 5 mL of medium. Positive control (positive control (PC), 100% lysis, 0.1% TritonX) shown in gray. n = 3 (independent samples). Data shown as mean ± standard deviation. b Corresponding cell viability of fibroblasts based on metabolic activity using an ATP dependent Cell Titer Glo assay. Metabolic activity values expressed relative to untreated control. n = 3 (independent samples). Data shown as mean ± standard deviation. c Attach-ment and interfacing of hydrogel sealant on tissue as showcased by H&E stained biopsy sec-tions of porcine stomach, small intestine and colon at two different magnifications. Histology samples were prepared in duplicate. Figure wide color coding: purple—double layer patch without mIPN, intense red—fully applied patch using PNAGA mIPN, blue—fully applied patch using PNAGA mIPN including a TurnON sensing element, turquoise green—fully applied patch using PNAGA mIPN including a TurnOFF sensing element, dark gray—positive control. Figure 4c has been created using biorender.com.

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