Fig. 1: Illustration of the click chemistry amplified nanopore (CAN) assay workflow for quantification of HIV-1 p24 antigen in human serum. | Nature Communications

Fig. 1: Illustration of the click chemistry amplified nanopore (CAN) assay workflow for quantification of HIV-1 p24 antigen in human serum.

From: A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples

Fig. 1

(1) blood samples were collected into serum separator tubes, and sera were collected after centrifugation and clot formation; (2) serum samples were incubated with capture antibody-modified magnetic beads (MBs) and detection antibody-modified copper oxide nanoparticles (CuONPs) to form a sandwich structure between MBs, enriched p24 antigens and CuONPs; (3) sandwich complexes were magnetically separated, and Cu+ ions were released from them under acidic conditions; (4) DNA probes were formed by a Cu+ ion catalyzed click reaction and a modification with a pair of host-guest molecules; (5) finally, DNA probes were collected and subjected to single-channel recordings using an α-HL nanopore for p24 antigen quantification. The illustration is not drawn to scale. (Created with BioRender.com).

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