Fig. 7: In vivo antitumor effect and toxicity of PTPR.

a The injection schematic for PTPR and in vivo antitumor effect analysis. b Xenograft mouse model established using EO771 cells in C57BL/6 mice injected with PTPR or Vehicle (n = 5 mice per group). In vivo generated tumors are depicted. c, d Analysis of tumor weight (c) and growth (d) in the xenograft mouse model. Data are presented as mean  ± SEM of n = 5 mice per group. Two-sided Mann-Whitney test for (c) and Two-way ANOVA for (d). e Survival in the mice injected with PTPR or vehicle. n = 10 mice per group. Log-rank test. f Representative IHC staining in randomly selected tumors from mice treated with PTPR or vehicle. Scale bar: 100 µm. g–i Flow cytometric analysis with median fluorescence intensity (MFI) of PD-L1 (g), CD3⁺ CD8⁺ T cells (h) or granzyme B-positive CD3⁺ CD8⁺ T cells (i). Data are presented as a box plot with box and whiskers. Bounds of box show the 25th and 75th percentiles, and the central lines in the box represent the median value. Whiskers show min to max value, n = 5 per group. Two-sided Mann-Whitney test. j The injection schematic for PTPR and αCTLA-4 in vivo antitumor effect analysis. k Xenograft mouse model established using EO771 cells in C57BL/6 mice with indicated treatment (n = 5 mice per group). In vivo generated tumors are depicted. l, m Analysis of tumor growth (l) and weight (m) in the xenograft mouse model. Data are presented as mean  ± SEM of n = 5 mice per group. Two-way ANOVA and One-way ANOVA followed by Tukey test. n, o Flow cytometric analysis of CD3⁺ CD8⁺ T cells (n) or granzyme B-positive CD3⁺ CD8⁺ T cells (o). Data are presented as a box plot with box and whiskers. Bounds of box show the 25th and 75th percentiles, and the central lines in the box represent the median value. Whiskers show min to max value, n = 5 per group. One-way ANOVA followed by Tukey’s test. p Survival in the mice with indicated treatment. n = 10 mice per group. Log-rank test. q A schematic model of TMUB1 regulation of PD-L1 and HUWE1.