Fig. 2: G1 replisomes differ quantitatively in subunit composition from S-phase replisomes. | Nature Communications

Fig. 2: G1 replisomes differ quantitatively in subunit composition from S-phase replisomes.

From: Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Fig. 2

a–c Replisomes in S- and G1-phase contain the same set of proteins. Cells were synchronously (a) released into S-phase, (b) arrested in S-phase using hydroxyurea (HU), or (c) G1 replication was induced for 3 h using the CDK/DDK bypass system. Replisomes were affinity-purified via GFP-tagged GINS-subunit Psf2 and analyzed by mass spectrometry. Volcano-plots show the enrichment of proteins in GFP-tagged samples versus untagged control samples. Colors indicate statistically significantly enriched proteins and replisome sub-complexes as indicated. Data from n = 3 biological replicates. d G1 replication induces fewer replisomes compared to S replication, and additionally polymerase α/primase association is reduced. Label-free quantification and comparison of the datasets shown in (a–c) using intensity-based absolute quantification (iBAQ). Boxes indicate the median with the first and third quartile of the sub-complexes, whiskers indicate the minimum and maximum (calculated by extending the box by 1.5 inter-quartile range). Mean iBAQ values for individual proteins are plotted as circles. Data from n = 3 biological replicates.

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