Fig. 1: CNTF-induced mitochondrial morphological changes in rod photoreceptors.

a Confocal microscopy images of P70 WT and rds retinas with the mitochondrial PhAM reporter activated in rod photoreceptors. b A dissociated P35 WT rod photoreceptor with PhAM reporter shown as Airyscan fluorescent image (left) and brightfield image (right). c SIM images show PhAM-labeled rod mitochondria in WT, rds, and rds mutant injected with LV-IG or LV-CNTF at P28 and harvested at P96. d TEM images of rod inner segments at P52 in WT, rds, and rds mutant injected with LV-CNTF. All image analyses used three independent retinas (N = 3). Scale bars: a 20 μm; b 10 μm; c 3 μm; d 0.5 μm. WT wild type, rds Prph2 P216L mutant, is inner segment, os outer segment, onl outer nuclear layer, opl outer plexiform layer, inl inner nuclear layer, gcl ganglion cell layer.