Fig. 2: Characterization of rod photoreceptor mitochondrial morphological features. | Nature Communications

Fig. 2: Characterization of rod photoreceptor mitochondrial morphological features.

From: Ciliary neurotrophic factor-mediated neuroprotection involves enhanced glycolysis and anabolism in degenerating mouse retinas

Fig. 2: Characterization of rod photoreceptor mitochondrial morphological features.The alternative text for this image may have been generated using AI.

a Quantification of PhAM-labeled mitochondria in rod inner segments captured with SIM in WT, rds, and rds retinas (N = 3) treated with LV-IG or LV-CNTF. Violin plots show geometrical features of the individual mitochondrion, including sphericity, compactness, ratio of surface area to volume (SA:V), and distribution isotropy. Dots represent individual mitochondria data points. Boxes represent 50% of the mitochondria, and yellow lines represent median values. The horizontal bounds of the box indicate 25th and 75th percentiles. The lower whiskers comprise the minimum data value within 1.5 times the interquartile range below the 25th percentile. The upper whiskers include the maximum value of the data within 1.5 times the interquartile range above the 75th percentile. One-way ANOVA and Tukey all-pairs test were applied with adjusted P values shown. P < 0.0001 is indicated as ****. b Principal component analysis (PCA) separates rod mitochondrial geometry. Separation in PCA1 is mainly driven by surface area, volume, and sphericity, while compactness and SA:V contribute to separation in PCA2. N in b represents the number of mitochondria used for the analysis shown in this figure.

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