Fig. 3: Influences on retinal cell respiration by the rds mutation and CNTF. | Nature Communications

Fig. 3: Influences on retinal cell respiration by the rds mutation and CNTF.

From: Ciliary neurotrophic factor-mediated neuroprotection involves enhanced glycolysis and anabolism in degenerating mouse retinas

Fig. 3: Influences on retinal cell respiration by the rds mutation and CNTF.The alternative text for this image may have been generated using AI.

a Seahorse metabolic flux analysis measuring basal OCR (left) and ECAR (right) for WT and rds retinas at P17, P25, and P35. For each age and genotype, N = 6 independent retinas were used. P values from the two-tailed student t-test are indicated. b Effects of CNTF on basal OCR and ECAR in rds retinas treated with LV-IG or LV-CNTF from P25-P35 compared to WT and non-treated rds retinas. Independent sample numbers N and adjusted P values from one-way ANOVA and Tukey all-pairs test are shown with P < 0.0001 indicated as ****. c, d Seahorse mitochondria stress tests to determine basal and maximum OCR, as well as ATP-linked OCR by sequential treatments with respiratory chain inhibitors oligomycin (oligo), uncoupling agent FCCP, antimycin A (AA), and rotenone (Rot) for WT, rds, and rds retinas treated with either LV-IG or LV-CNTF from P26-P42. c Seahorse assay tracings and the states indicating (1) basal OCR, (2) oligomycin inhibition of ATP synthase, (3) maximum OCR, and (4) total inhibition of respiration with AA and Rot. d Bar graphs show basal OCR, ATP-linked OCR, and maximal OCR. Independent sample numbers N and adjusted P values from two-way ANOVA and Tukey all-pairs test are shown. For a, b, d, data were presented as mean values ± SEM.

Back to article page