Fig. 6: Influence of CNTF on metabolic gene expression and enzyme activity. | Nature Communications

Fig. 6: Influence of CNTF on metabolic gene expression and enzyme activity.

From: Ciliary neurotrophic factor-mediated neuroprotection involves enhanced glycolysis and anabolism in degenerating mouse retinas

Fig. 6: Influence of CNTF on metabolic gene expression and enzyme activity.The alternative text for this image may have been generated using AI.

a–d Transcriptome analysis of WT retina and rds retinas treated with LV-IG or LV-CNTF from P25-P35. Heatmaps show relative transcript levels (average of N = 3, each N contains two retinas). a Glycolytic pathway enzymes and Mpc2. b Mitochondrial Pdha1, Pdhb, and TCA cycle enzymes. c Participants of mitochondrial biogenesis. d Nuclear genome encoded mitochondrial respiratory chain components. Asterisks indicate genes with decreased transcripts compared to WT. e Western blot analysis of retinal extracts from WT retina and rds retinas treated with LV-IG or LV-CNTF from P25-P100. Bar graphs show the quantification of CNTF signaling effector pY705 STAT3 and total STAT3, the active pY10 LDHa and LDHa. For e, data were presented as mean ± SEM. Independent retinal samples (N = 3) and adjusted P values based on one-way ANOVA and Tukey all-pairs test are indicated.

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