Fig. 7: Contribution of glycolysis and mitochondrial activities to retinal metabolism and CNTF-induced metabolic changes. | Nature Communications

Fig. 7: Contribution of glycolysis and mitochondrial activities to retinal metabolism and CNTF-induced metabolic changes.

From: Ciliary neurotrophic factor-mediated neuroprotection involves enhanced glycolysis and anabolism in degenerating mouse retinas

Fig. 7: Contribution of glycolysis and mitochondrial activities to retinal metabolism and CNTF-induced metabolic changes.The alternative text for this image may have been generated using AI.

a Schematic illustration of the glycolytic pathway and TCA cycle with glucose as a fuel. Enzymatic steps affected by specific inhibitors are indicated (red circle). b–d Metabolomics heatmaps show the effects of inhibitors in WT, rds, and rds retinas treated with LV-IG or LV-CNTF on metabolites. b amino acids. c fatty acid synthesis intermediates. d energy currency-related metabolites. e–m Bar graphs show the effects of inhibitors on the levels of ATP (e, h), lactate in the tissue (i), acetyl-CoA (f, k), TCA cycle metabolites (g, j, l), and GSH (m). Viral vector treatment periods are shown below treated rds samples. For e–m, data were presented as mean ± SEM. Independent sample numbers (N) are indicated within the bars. Two-way ANOVA and Tukey’s multiple comparison test were applied to the entire group. For clarity, only significant adjusted P values for pairs of samples with and without a given inhibitor are shown with P < 0.0001 indicated as ****. See Source Data for the entire statistical analysis.

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