Fig. 2: salm regulates conversion of muscle fiber contractile type and mitochondrial network orientation.
From: Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
a Adult wild-type flight muscles (fibrillar) stained for F-actin (phTRITC). b salm KD muscle fiber stained for muscles showing tubular muscle type (Scale Bars: 100 μm). c, d, e Wildtype flight muscles show elongated, parallel mitochondria (mito-gfp) between myofibers (phTRITC). f, g, h The knockdown of salm (UAS-salm RNAi;UAS-mito-gfp;mef2) in flight muscles results in fiber conversion to tubular muscle type and mitochondria to a grid-like network. (i) Wild type leg muscles show tubular muscle type in the coxa. Inset shows a cross-section of leg muscles displaying well-aligned fibers. j, k Wildtype leg muscles show parallel mitochondria (mito-mcherry) aligned next to myofibrils (phTRITC). l salm OE converts muscle fibers to fibrillar in nature. Inset, well-defined fibrillar fibers in cross-section of leg muscles. m, n salm OE leg muscles have parallel mitochondrial networks (mito-mcherry) along the myofibrils (phTRITC) (Scale Bars: 5 μm for all). o Quantification of mitochondrial network orientation. Dotted line represents parallel equal to perpendicular. mito-gfp;mito-mcherry;mef2-Gal4 used as Wildtype, WT. (WT-IFM, n = 9 animals; salm-KD IFM, n = 7 animals; WT-Leg Fiber I, n = 6 animals; salm-OE Leg Fiber I, n = 8 animals). Each point represents value for each animal dataset. Bars represent mean ± SD. Significance determined as p < 0.05 from one way ANOVA with Tukey’s (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001; ns, non-significant).
