Fig. 5: H15 is downstream of salm and H15 OE rescues fiber-type switching in salm KD fibers.
From: Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
a–d Wildtype fibrillar IFM stained for F-actin (phTRITC), nuclei (DAPI), and Salm antibody showing Salm expression in the nuclei. e–h salm KD IFM showing decreased expression of salm and fiber type switch in IFM from fibrillar to tubular. i–l H15 OE; salm KD shows fibrillar fiber type in IFMs and decreased Salm antibody immunofluorescence (Scale Bars: 5 μm for all). m Quantification of fluorescence intensity of Salm antibody staining (Wildtype, n = 12, 3 animals; salm KD, n = 11, 3 animals; H15 OE; salm KD, n = 11, 4 animals). Each point represents the value for each dataset (Wildtype, n = 16; salm KD (UAS-salm RNAi;UAS-mito-gfp;mef2) (, n = 20 animals; H15 OE; salm KD, n = 26 animals). n Quantification of fiber type in IFMs (Wildtype, n = 16; salm KD (UAS-salm RNAi;UAS-mito-gfp;mef2) (n = 20; H15 OE; salm KD, n = 26). o Wildtype IFM showing a parallel mitochondrial network (MitoTracker). p Knockdown of salm (UAS-salm RNAi;UAS-mito-gfp;mef2) in flight muscles coverts parallel mitochondrial arrangement to a grid-like network. q H15 OE; salm KD shows parallel mitochondrial networks in IFMs similar to wildtype (Scale Bars: 5 μm for all). r Quantification of mitochondrial network orientation. Dotted line represents parallel equal to perpendicular (WT IFM, n = 3 animals; salm KD IFM, n = 3 animals; H15 KD; salm OE IFM, n = 3 animals). Each point represents value for each animal dataset. Bars represent mean ± SD. Significance determined as p < 0.05 from one way ANOVA with Tukey’s (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001; ns, non-significant).
