Fig. 6: cut regulates conversion of mitochondrial networks configuration, but not contractile type, in Fiber II of Drosophila leg muscles.
From: Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
a Adult wildtype leg coxa muscles showing distinct parallel and grid-like mitochondrial networks (mito-gfp) in Fiber I, II, and III (demarcated by dotted lines). b cut KD converts grid-like mitochondrial networks to parallel in Fiber II (Scale Bars: 20 μm). c, d Wildtype leg Fiber I showing parallel mitochondrial networks (mito-GFP) and tubular muscle fiber (phTRITC). e, f Wildtype leg Fiber II and g, h Fiber III showing grid-like mitochondrial networks (mito-GFP) and tubular muscle fiber (phTRITC). i, j cut KD Fiber I showing parallel mitochondrial networks (mito-GFP) and tubular muscle fiber (phTRITC). k, l cut KD Fiber II showing parallel mitochondrial networks (mito-GFP), unlike wildtype Fiber II, while fibers remain tubular (phTRITC). m, n cut KD Fiber III showing grid-like mitochondrial networks (mito-GFP) and tubular muscle fibers (phTRITC). (Scale Bars: 5 μm for all). o Quantification of mitochondrial network orientation. Dotted line represents parallel equal to perpendicular. mito-gfp;mito-mcherry;mef2-Gal4 used as Wildtype, WT (WT-Fiber I, n = 4 animals; cut KD-Fiber I, n = 4 animals; WT-Fiber II, n = 3 animals; cut KD-Fiber II, n = 6 animals; WT-Fiber III, n = 4 animals; cut KD-Fiber III, n = 4 animals). p Mitochondrial volume as a percent of total muscle volume. UAS-mito-gfp;UAS-mitoOMM-mcherry;mef2-Gal4 used as wildtype (WT-Leg Fiber I, n = 4 animals; cut KD-Leg Fiber I, n = 7 animals; WT-Leg Fiber II, n = 4 animals; cut KD-Leg Fiber II, n = 10 animals; WT-Leg Fiber III, n = 5 animals; cut KD-Leg Fiber III, n = 5 animals). Each point represents value for each animal dataset. Bars represent mean ± SD. Significance determined as p < 0.05 from one way ANOVA with Tukey’s (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001; ns, non-significant).
