Fig. 7: cut is a repressor of salm and cut KD rescues fiber-type switching and conversion of mitochondrial network organization in salm KD fibers.
From: Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
a, b, c Fibrillar flight muscles (IFMs) stained for F-actin (phTRITC) and mitochondria (MitoTracker) showing parallel aligned mitochondria between myofibrils. d, e, f salm KD (UAS-salm RNAi;UAS-mito-gfp;mef2) shows fibrillar muscles switched to tubular muscle type and mitochondria (mito-gfp) converted to grid-like networks. g, h, i salm KD; cut KD shows fibrillar fiber type and parallel mitochondrial networks (MitoTracker) in IFMs similar to wildtype. j, k, l cut-OE shows tubular fiber type and grid-like mitochondrial networks (MitoTracker) in IFMs similar to salm KD (UAS-salm RNAi;UAS-mito-gfp;mef2) (Scale Bars: 5 μm for all). m Quantification of mitochondrial network orientation. Dotted line represents parallel equal to perpendicular (WT IFM, n = 3 animals; salm KD(UAS-salm RNAi;UAS-mito-gfp;mef2) IFM, n = 3 animals; salm KD; cut KD IFM, n = 3 animals; cut-OE, n = 4 animals). n Wildtype fibrillar IFM stained for nuclei (DAPI), and salm antibody showing salm expression in the nuclei. o salm KD IFM showing decreased salm expression. p cut KD IFM and q salm KD; cut KD showing restored salm expression in the nuclei. r cut-OE showing absence of salm expression in the nuclei (Scale Bars: 5 μm for all). s Quantification of salm transcript levels (WT IFM, n = 5; salm KD (UAS-salm RNAi;UAS-mito-gfp;mef2) IFM, n = 5; cut KD, n = 3; salm KD; cut KD IFM, n = 3). t Quantification(qPCR) of cut, salm, and H15 transcript levels. Each point represents value for each dataset. Bars represent mean ± SD. Significance determined as p < 0.05 from one way ANOVA with Tukey’s (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001; ns, non-significant).
