Table 1 Normalized abundance in percentage of MreBs and Fib in Mcap transformants

	Passage^a	MreB1	MreB2	MreB3	MreB4	MreB5	Fib
Mcap^control	4 P	ND	ND	ND	ND	ND	ND
Mcap^mreB1-5 (cl. 7-5)	4 P	0.51 ± 0.07	0.19 ± 0.03	0.34 ± 0.01	0.03 ± 0.01	1.47 ± 0.15	ND
Mcap^mreB1-5 (cl. 7-5)	8 P	0.47 ± 0.06	0.11 ± 0.03	0.18 ± 0.003	0.03 ± 0.02	1.45 ± 0.015	ND
Mcap^mreB1-5-fib (cl. 8-7)	4 P	0.48 ± 0.11	ND	ND	0.20 ± 0.02	1.97 ± 0.037	0.28 ± 0.05
	4 P	0.52 ± 0.07	ND	ND	0.24 ± 0.07	2.26 ± 0.051	0.34 ± 0.04
	8 P	0.14 ± 0.01	ND	ND	0.07 ± 0.004	0.56 ± 0.03	ND
Mcap^mreB1-5-fib (cl. 32-1)	4 P	0.80 ± 0.15	0.30 ± 0.02	ND	ND	2.02 ± 0.51	0.36 ± 0.02
	4 P	0.68 ± 0.10	0.20 ± 0.05	ND	ND	1.60 ± 0.25	0.30 ± 0.02
	6 P	0.11 ± 0.01	ND	ND	0.04 ± 0.03	0.38 ± 0.04	ND

^a Proteins were detected in bacteria subcultured 4, 6 or 8 (4 P, 6 P, 8 P) times after transformation in axenic medium (4, 6 or 8 passages). For Mcap^mreb1-5-fib 4 P (clones 8-7 and 32-1), two biological replicates were analyzed. Values are expressed as mean ± SD, with three technical replicates for each clone carrying a combination of cytoskeleton genes. The absence of MreB and Fib in Mcap^control was checked on two technical replicates. ND: Not detected.

Quick links

Search