Fig. 6: pTINCR is induced by damage in a p53 dependent manner and it is required for damage-induced differentiation.

a TINCR lncRNA expression analyzed by RT-qPCR after 24 h of treatment with doxorubicin, actinomycin D or nutlin-3a in the indicated cell lines. mRNA expression is normalized to GAPDH in each case and relative to the control. Error bars represent the mean ± SD of N = 3 technical replicates from a representative experiment performed twice (hSCC10 and HCT116) or 3 times (A549) independently with similar results. b Endogenous pTINCR expression analyzed by immunofluorescence in the indicated cell lines after 24 h of treatment with doxorubicin. Representative images are shown, the experiment was performed twice independently. Scale bars correspond to 50 µm. c TINCR lncRNA expression levels analyzed by RT-qPCR after 24 h of doxorubicin in WT, E6-transduced or pTINCR-KO MCF7 cells. mRNA expression is normalized to GAPDH in each case and relative to the control. Error bars represent the mean ± SD of N = 3 technical replicates from a representative experiment performed 3 times independently with similar results. n.s: not significant. Multiple T-TEST corrected for multiple comparison was performed. d Endogenous pTINCR expression analyzed by immunofluorescence after 24 h of exposure to UV light in WT, E6-transduced or pTINCR-KO MCF7 cells. Representative images are shown, the experiment was performed twice independently. Scale bars correspond to 50 µm. e Expression of the indicated differentiation markers after 24 h of treatment with doxorubicin in WT, E6-transduced, pTINCR-KO or pTINCR-KO re-expressing pTINCR-HA (pTINCR KO + OE) MCF7 cells. mRNA expression is normalized to GAPDH and relative to the control in each case. Error bars represent the mean ± SD of N = 3 technical replicates from a representative experiment performed 3 times independently with similar results. n.s: not significant. Multiple T-TEST corrected for multiple comparison was performed. Source data are provided as a Source Data file.